Development of a Yeast Inhibitive Assay for Anionic Heavy Metals Using the 1-(4,5-Dimethylthiazol-2-Yl)-3,5-Diphenyltetrazolium Bromide (MTT) Assay

Currently, heavy metals pollution is significantly becoming a global concern as it causes severe toxicity towards human health and the environmental condition. Intensive efforts to develop highly sensitive, rapid, simple assay assess toxic effects of in aquatic bodies have been done. An emerging tool solve this matter by using microorganisms one them yeast. In study, simple, cost-efficient Baker’s yeast (Saccharomyces cerevisiae) respiration with tetrazolium dye (MTT) developed potential biomonitoring tool. To achieve high sensitivity, optimization one-factor-at-a-time (OFAT) was first carried out. The best conditions giving optimum response occurred at pH 5.8 30 min contact time. Molybdate chromate exhibited exponential decay type inhibition curves calculated half maximal inhibitory concentration, IC50 1.137 mg/L for molybdate 1.247 chromate. Limits detection (LOD) were 0.313 chromate, respectively. newly can help monitoring rivers agricultural areas.